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Two independent reviewers performed the study selection process and any disagreements were resolved by a third reviewer
Signs of Degradation Inspect the solution before each use: Clear and colorless to slightly off-white: Normal, proceed with use Cloudy or milky: Do not use precipitation or aggregation has occurred Visible floating particles: Do not use particulate matter indicates degradation or contamination Yellow or brown discoloration: Do not use oxidative degradation has likely occurred If in doubt, discard and reconstitute a fresh aliquot from lyophilized stock
Cardiac blood samples were collected in heparinized tubes and centrifugated (1,500 g, 10 min, 4 C)
