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The gel purified digested His6-AV-Ubiquitin- semapeptide (nucleotide sequence) encoding gene PCR product was ligated into Ndel and Xhol sites of pET24 plasmid vector using Quick ligation kit (NEB)
opened multi-dose pens have product-specific in-use periods (e.g., Ozempic 6 weeks, Victoza/Saxenda 1 month, Byetta 30 days)
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