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One milliliter of culture was then back-diluted again into each of two flasks containing (1) 49 ml of DRM supplemented with 50 g ml 1 carbenicillin, 50 g ml 1 spectinomycin, 20 M arabinose and 20 g ml 1 chloramphenicol or (2) 49 ml of DRM supplemented with 50 g ml 1 carbenicillin, 50 g ml 1 spectinomycin and 20 M arabinose

However, chronic GH treatment carries substantial risks, including glucose intolerance, insulin resistance, diabetes, cancer, and cardiovascular complications