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After washing, cells were dispensed into 96-well plates containing 11 mM glucose the indicated concentration of agonist and incubated for 16-18 hours
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The acylation process, which confers semaglutide with an extended half-life, also substantially increases its molecular size, a factor that is generally anticipated to impede its passage through the tightly regulated BBB
In the other enzymatic reaction, methylcobalamin supports the methionine synthase reaction, which is essential for normal metabolism of folate
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