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A different GLP-1R antibody (AP31362PU-N, Acris Antibodies GmbH, Herford, Germany), was used at a final titre of 1:100, and a cocktail of monoclonal antibodies to the phosphorylated and non-phosphorylated Neurofilaments of size 22 kDa (Clone N52, Sigma-Aldrich, Dorset, UK) and the 57 kDa type III filament, Peripherin (Novocastra Laboratories, Newcastle, UK) were used at final titres of 1:20,000 and 1:500 respectively, as structural neuronal markers for the frozen post-fixed sections
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