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"GlyNAC supplementation in old mice improves brain glutathione deficiency, oxidative stress, glucose uptake, mitochondrial dysfunction." J Gerontol A Biol Sci Med Sci

Alkylation, reduction and labeling are usually performed on cell lysates and though protein extraction is performed using acidic or sometimes anaerobic conditions, it does not entirely preclude that modifications of the cysteine redox state occur during the procedure or that the modifications are insufficiently trapped