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After TEV digestion overnight at 4 C, the elution was then subjected to Strep-Tactin Sepharose resin (IBA, 2-1201-010), washed with wash buffer and eluted with wash buffer supplemented with 10 mM desthiobiotin (Sigma-Aldrich, D1411)
Journal of Diabetes Research, 2022, 1-12
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Using this phenotypical characterization, most of FoB and MZB are thought to derive from TrB1 that have transitioned to TrB2 61,62
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