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[22] [23] Baseline assessment should be considered before initiating tirzepatide in high-risk patients, including those over 65 years of age, individuals with pre-existing gastrointestinal disorders, patients on concurrent metformin or acid-suppressing medications, and those following restrictive diets

Plasma was isolated by centrifugation at 17,000 g for 5 min at 4 C and diluted 1:10 in 50% (v/v) glycerol in PBS (pH 7.4) (Sigma-Aldrich) for storage at 20 C until analysis

PMID: 16181611

Notably, the disease group exhibits a significantly elevated histological score in comparison to the normal group, reflecting an increase in cortical neurons with rounded vesicular nuclei and neuropil exhibiting sharply demarcated nuclei or signs of meningeal involvement (Syed et al., 2021