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or anti--Actin mAb, 0.5 g/ml, Sigma-Aldrich, Toronto, CA) in 5% milk/PBS, followed by several washes in PBS and incubation with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5000 v/v, BioRad, Ont., CA)
One hypothesis from researchers at Harvard Medical School: chronic NNMT suppression might alter methylation donor availability, since NNMT consumes S-adenosylmethionine (SAM) as a methyl donor
This ensures every vial meets global standards for sterility, purity, and potency
However, it usually self-seeds in the vicinity, so take care to keep the plants under control
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