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Inhibition and covalent modification of tyrosine hydroxylase by 3,4-dihydroxyphenylacetaldehyde, a toxic dopamine metabolite
The direct injection ensures that the body absorbs the entire B12 dose, offering a more potent and immediate impact compared to other supplementation methods
Afterwards, the sections were washed with Triton X (0.1%) three times (10 min each), followed by incubation with the secondary antibodies conjugated and diluted in the blocking buffer at room temperature for 2 h (see Supplementary Table 4 for secondary antibodies used in this study)
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