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The main fermentation was conducted in 100 mL of YCK50D medium (Cha et al., 2004) containing 30 g/L yeast extract, 5 mM cysteine, 0.6 g/L KH 2 PO 4 , and 50 g/L glucose
It passed a skin-irritation test and is free from 20 potentially irritating ingredients
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