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The resolved gels were transferred from the pre-cast SDS-PAGE gels to polyvinylidenefluoride (PVDF) membranes, and blocking was performed with 5% bovine serum albumin (BSA) in Tris-buffered saline (TBS-T) containing 0.05% Tween-20 and incubated with the primary antibodies: MOTS-c antibody (MOTSC-101AP, FabGennix)
The harsh high-altitude environment, characterized by low ambient oxygen and low temperatures, can exacerbate preexisting lung diseases such as chronic bronchitis, emphysema, asthma, and obstructive lung disease, leading to hypoxemia and secondary polycythemia
Because glutathione helps manage oxidative stress, NAC has been studied in several areas of health
It reached Phase II clinical trials for postoperative ileus (a gut-recovery condition) and was discontinued after the trial did not show efficacy
