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RNA-seq Total RNA was isolated using the RNeasy kit (Qiagen) from three independent replicates of MDA-MB-231 cells incubated for 5 h with ML162 (125 nM) or ESA (100 M) and two replicates from the control incubated with the vehicle for ESA (methanol)

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Use a seven-day moving average rather than individual data points

Perhaps you were treated for a persistent skin condition, a chest infection, or a tick bite, and while the primary infection cleared up, you were left with a new set of "mystery symptoms"bloating, changes in bowel habits, or a lingering sense of fatigue