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Subsequently, correct insertion of the mutant gene fragments into the marker free gshA mutant were checked using appropriate forward and reverse primers of the respective genes in combination with the k1 and k2 KEIO primers listed in Table 3 using colony PCR

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We investigated changes in gene expression profiles and small molecular metabolites in HBE cells induced by Al 2 O 3 NPs

During the screening process, 32 patients were excluded because of severe comorbidities,25 patients with history of malignancy were excluded and another 30 were excluded due to incomplete follow-up data