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In heterodimeric GST complexes such as those formed by the cytosolic mu and alpha classes, however, the cleft between the two subunits is home to an additional high-affinity nonsubstrate xenobiotic binding site, which may account for the enzymes' ability to form heterodimers
However, these are the only option for higher-volume draws
When cells reached 80% confluence, the monolayer was rinsed 3x with PBS and cultured in phenol red-free MEM without additives (no FBS, L-glutamine, ITS, or estradiol)
Taking hCG while drastically cutting calories may cause more harm than benefit
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