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Although QVD inhibits caspase-1, it did not fully suppress 7-AAD + lytic death in our assays
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controlling the fermentation process: controlling the dissolved oxygen range to be 15-30% by controlling the rotating speed and/or introducing pure oxygen in the fermentation process, and controlling the dissolved oxygen range to be OD 600 When the value is increased to 160, adding 1.0mM IPTG for induction, reducing the temperature to 30 +/-1.0 within 15-30min after induction, and carrying out induction culture for 24 h
Nature 541 , 481487 (2017)
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