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M.Ghavimi-AlaghaD.StarrS
The protein was transferred to 0.2 m pore-sized nitrocellulose (wet transfer, 1 hour, 100 mA, RT) and then blocked with 5% non-fat dry milk in TBS-T (20 mmol/L TrisHCl, 150 mmol/L NaCl, 0.05% Tween 20, adjusted to pH 7.4) and incubated with the following primary monoclonal antibodies: anti--actin, anti-cleaved-PARP, anti-cleaved Caspase-3, anti-cleaved Caspase-9, and anti-COX IV (for apoptosis evaluation) or anti-LC3A/B, anti-Beclin-1, anti-Atg12, and anti-Atg-7 (for autophagy evaluation) antibodies, diluted 1:1,000 in blocking buffer overnight at 4C
[8] Higher concentrations can be safely applied to clothing, although it may damage some types of synthetic fibers
IV Therapy, short for Intravenous Therapy, involves administering fluids, vitamins, minerals, and other nutrients directly into the bloodstream through a vein
