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For these assays, SUP-B15 cells were cultured in Iscoves modified Dulbeccos medium supplemented with 4 mM L-glutamine, 1.5 g l 1 sodium bicarbonate, 0.05 mM 2-mercaptoethanol, 80% RPMI-1640 and 5% FBS instead of 20% FBS to improve toxin activity
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The combination can affect all of these, and catching abnormalities early prevents complications
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