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Proteomics sample preparation Frozen tissue samples were homogenized in 0.1 M Tris-HCl, pH 7.6 and 2% SDC, heated for 5 min at 95 C and sonicated (Diagenode Bioruptor at high intensity, 15 30 s)
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Cell Metab 27(4):740756 Buchan AM, Polak JM, Capella C, Solcia E, Pearse AG (1978) Electronimmunocytochemical evidence for the K cell localization of gastric inhibitory polypeptide (GIP) in man
The reason for it is when you start to look at the patients journey, obesity is a chronic disease
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