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Flies were then washed (twice for 1 min each) with 0.15 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 on ice and were reacted with 2.5 mM Ce-DAB2 solution in cacodylate buffer 36 for 75 min to produce the desired brown intensity color from the precipitate
The injection Hydroxocobalamin is given as an intramuscular injection (into a muscle), typically by a trained healthcare professional
doi: 10.1530/joe-18-0374 28 YamamotoCMiyoshiHOnoKSugawaraHKamedaRIchiyamaMet al
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