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Reverse transcription and real-time quantitative PCR (Q-PCR) Total RNA was isolated from frozen liver pieces using Tri-Reagent (Molecular Research Center, Inc.) according to manufacturers protocol
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Results Long-term BDNF treatment improves excitatory synaptic current frequency and amplitude To investigate the ability of prolonged exposure to BDNF to regulate network dynamics and synaptic transmission, dissociated hippocampal cultures were treated for 4 days with 20 nM mature BDNF (named BDNF-treated cultures) and compared to untreated ones (named Controls)
