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For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min

As detailed in the preceding sections, SFNs bioavailability and effects are significantly modulated by key host factors

Presented for research literature review only Anand P et al

"Higher concentration always works better" Maquart's 1988 paper demonstrated collagen stimulation at picomolar to nanomolar concentrations 6