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doi:10.1111/cns.13378 104
The GST-fusion protein first binds to glutathione agarose, and then after other contaminating proteins have been washed away, reduced glutathione is added to elute the GST-fusion protein from the resin (Figure 5)
the solution is further diluted into 250 ml or 500 ml with normal saline or 5% glucose solution for intravenous infusion respectively
Rubin J, Hameed B, Gottfried M, Lee W, Sarkar M
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