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Real-time quantitative polymerase chain reaction amplification was done utilizing PowerUp SYBRTM Green Master Mix (Thermo Fisher Scientific), and data were analyzed with an Applied Biosystems and software version 3.1 (Thermo Fisher Scientific, MA, USA)

It is usually injected once a week into the fatty tissue just under the skin, often in the stomach, thigh, or upper arm

Because deep sleep is one of the most restorative phases of our sleep architecture, DSIP is of interest for those seeking to improve sleep quality, recovery, and even manage stress-induced interruptions in sleep patterns, promoting stress reduction

Median duration of gastrointestinal side effects on semaglutide 2.4 mg: nausea 8 days, vomiting 2 days, diarrhoea 3 days, constipation 47 days