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Mice and rats were anesthetized with subcutaneous ketamine/xylazine, the corneal surface was numbed with 0.5% proparacaine drops, and pupils were dilated with sequential drops of 0.5% tropicamide and 2.5% phenylephrine HCl
(River Peptides) When immune clearance of senescent cells declines with age, their accumulation accelerates organ dysfunction, frailty, age-related disease (cardiovascular disease, osteoarthritis, kidney decline, endocrine decline, etc)
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RNA extraction, RT-PCR, and real-time PCR Total RNA extraction was performed using the Maxime RT Premix Kit (iNtRON Biotechnology) according to the manufacturers instructions
