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All reagents and solvents were obtained from commercial sources except for the protected pentafluorophenyl esters: Z-Trp-OPfp, Z-D-Phe-OPfp, Z-His( T Boc)-OPfp, Z-Lys( Boc)-NH2, and Z-Asp(O t Bu)OPfp, which were prepared in-house according to the well established procedures [31,32]
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After obtaining spectral libraries for bright light images of unlabeled tissue and neuromelanin and fluorescent images of fluorophores and background autofluorescence, brightfield and fluorescent images were "unmixed" into individual signal components (i.e
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