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Live-cell imaging systems enable real-time quantification of cellular dynamics under various treatment conditions
5: Semax Proposed use: Cerebral ischemia (shortage of blood flow and oxygen to the brain), migraine and trigeminal neuralgia (chronic pain condition in the face) Proposed form: Intranasal spray, subcutaneous injection CLICK HERE TO SIGN UP FOR OUR HEALTH NEWSLETTER No
We therefore sought a strategy to reduce the scale of the library to remain within the quantitative throughput limits of our assay (up to ~40,000 substrates), while still assaying a full repertoire of sequences likely to be cleaved by our evolved proteases (Extended Data Fig
Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]
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