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To quantify SNAP/FLAG-hGLP-1R association with clathrin, circular ROIs surrounding individual well defined puncta of fluorescence (5-50 per cell) were selected on CLC-GFP images and transferred to the corresponding SNAP ones using a Fiji macro and average fluorescence per pixel was measured in a central circle (c) of diameter 5 pixels (0.55 m) at each puncta location, in a surrounding annulus (a) with an outer diameter of 7 pixels (0.77 m), and in an area not containing any cell as background (bg)
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For type 2 diabetes, Medicare Part D plans often provide coverage for Ozempic and Rybelsus , though weight-loss use is excluded since Medicare legally cant cover weight loss medications
They are playing a numbers game, and coverage is granted when the cost of the medication is projected to be less than the future cost of treating these associated diseases
