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Clin Diagn Lab Immunol (2004) 11(1):195202
[16] [121] Upon leaving the stomach the hydrochloric acid of the chyme is neutralized in the duodenum by bicarbonate, [122] and pancreatic proteases release the vitamin from HC, making it available to be bound by IF, which is a protein secreted by gastric parietal cells in response to the presence of food in the stomach
The rate of flow through the column should not exceed 12ml/minute for every 1ml of column volume
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