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There are more nerves in the muscle in the thigh than in the deltoid muscle, and it is easier to find the safe place to do the injection in the upper arm (which is why this is a common used place to give vaccinations i.m.)

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Presented for research literature review only Di Simplicio P (1982)

At 48 hpi, the cells were fixed, permeabilised, and immunolabelled with the appropriate antibodies, and then processed for immunofluorescence microscopy