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By neutralizing ROS that would otherwise damage lipids, proteins, DNA, and transcriptional regulation, melatonin normally limits oxidative injury
For proliferation analysis, expanded HBs were fixed with 4% PFA, incubated in blocking and permeabilizing buffers (0.1% Triton X-100, 5% normal donkey serum), and then incubated with APC-conjugated Ki67 and other indicated antibodies [1]
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