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Cancer cells reprogram iron handling to sustain rapid proliferation, leading to expansion of the intracellular labile iron pool (LIP), composed largely of redox-active ferrous iron (Fe 2+ ) (67, 68)

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To achieve this, lipid-based fluorescent probes combined with imaging modalities, such as near-infrared live imaging, photoacoustic imaging, or mass spectrometry imaging, allow for the dynamic localization and quantification of PRLMs in vivo or in ex vivo tissues

~100:1 The typical GSH:GSSG ratio in normal cellular conditions heavily weighted toward the reduced form Under typical cellular conditions, the ratio of reduced glutathione (GSH) to oxidised glutathione (GSSG) is described in the literature as approximately 100:1 or higher in typical cells