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Commercial GHK-Cu serums typically claim 6-12 month shelf life unopened when properly formulated with preservatives and appropriate pH, dropping to 3-6 months after opening due to air exposure and contamination introduction

observed in Nature Medicine , NNMT inhibition in adipose tissue produced favorable body composition changes without negatively impacting muscle mass (Kraus et al., Nature Medicine )

Preparation of cigarette smoke extract CSE was prepared according to the previous study (Chen et al., 2009)

Also, the previous literature dominated by older trials from specific regions (mostly Europe), which may limit generalizability to other settings