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2 Methods 2.1 Animals and procedures The animal experimental procedures were approved by the Animal Care and Use Committee of Oujiang Laboratory (SYXK2023-0047, OJLAB22121609), and were carried out in strict accordance with the National Institutes of Health Guidelines for the Use of Laboratory Animals

doi: 10.1016/0192-0561(93)90031-S 129

Glutathione itself has poor oral bioavailability, but precursor amino acids (cysteine, glycine, glutamate) and cofactor nutrients are better absorbed

Intramuscular and cardiac concentrations of anserine are affected by a number of factors, including muscle fiber type, muscular contractility, and health status