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Copper toxicity: A theoretical concern given the copper content
Medical necessity must be documented for reconstructive procedures to secure payment
Following clearance by centrifugation, protein concentration of lysates was determined using a Pierce BCA protein assay (23225, Fisher Scientific) and 30 g of lysate was prepared using 4 LDS sample buffer (Thermo Fisher) and 10% 2-mercaptoethanol and run on NuPAGE 415% BisTris gels (Thermo Fisher)
Even though the test is widely available, most medical experts dont recommend that you use it
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