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RT-qPCR was carried out using Luna Universal One-Step RT-qPCR Kit (Biok E3005E) with the following primers: Gclc forward (F) GGCTCTCTGCACCATCACTT and reverse (R) GTTAGAGTACCGAAGCGGGG

This is because B12 acts on the bone marrow to normalize red blood cell creation, and the life cycle of a red blood cell is 90 days

Figure 2: Categorical weight loss of either 5%, 10%, or 15% in the randomly assigned treatment groups administered either 0.3 mg of cagrilintide, 0.6 mg of cagrilintide, 1.2 mg of cagrilintide, 2.5 mg of cagrilintide, 4.5 mg of cagrilintide, 3.0 mg of liraglutide, or a placebo

References 1 GensollenTIyerSSKasperDLBlumbergRS