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Body temperature was controlled at around 37 C using a temperature-controlled heating pad system (FHC Inc., USA)
As expected CO23, CO23RFLC and CO23RFK strains had an identical electrophoretic kariotype, as determined by pulse-field gel electrophoresis (PFGE), thus demonstrating that there was no selection of a contaminating FLC and MCFG resistant yeast during the process of resistance acquisition [15]
Injections may have higher initial costs but offer quick and potent benefits
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