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2019;316(2):C135c153
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Lancet Glob
Peptides were separated by chromatography with the following parameters: Acclaim PepMap100 C18 pre-column (2 cm, 75 m i.d., 3 m, 100 ), Pepmap-RSLC Proxeon C18 column (75 cm, 75 m i.d., 2 m, 100 ), 300 nl/min flow rate, using a gradient rising from 95% solvent A (water, 0.1% formic acid) to 40% B (80% acetonitrile, 0.1% formic acid) in 120 min, followed by a column regeneration of 20 min, for a total run of 140 min
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