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Enzyme samples were incubated with phosphate buffer, which included the appropriate concentrations of glutathione (GSH) and peroxide as substrates, to determine Gpx activity
Subsequently, 1 M dihydroethidium (DHE) was added to the medium and further incubated for 30 min
However, to make the most of your treatment and avoid complications, its essential to follow some key guidelines
#S8292, Selleck, Shanghai, China), an ASK1 inhibitor, was mixed with 1 mL of dimethyl sulfoxide (DMSO) to prepare the 10 mM mother liquor
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