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After TEV digestion overnight at 4 C, the elution was then subjected to Strep-Tactin Sepharose resin (IBA, 2-1201-010), washed with wash buffer and eluted with wash buffer supplemented with 10 mM desthiobiotin (Sigma-Aldrich, D1411)
A simple concentration makes the rest of the research workflow easier
(ClinicalTrialsgov, U.S
A systematic review found associations between circulating MOTS-c and metabolic states, including diabetes and obesity patterns [8]
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