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Each freeze-thaw cycle stresses the peptide in solution and can cause aggregation, the process by which peptide molecules clump together and lose biological activity
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PubChem Compound Summary for CID 3476, Glimepiride
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If I have any of this wrong, feel free to correct me (addressing more experienced members of this forum) The problem with a retest at this point is that after 6 injections, the B12 level will likely be elevated and it will be very difficult to know what the original value would have been
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