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After 24 h, expression levels of mutants were measured to be 90%100% of the wild-type for follow-up experiments
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Peptides can degrade more quickly under high temperatures, repeated freeze-thaw cycles, contamination, exposure to UV light, as well as improper pH, thus reducing shelf life
For general prevention in individuals at risk of deficiencysuch as vegans and vegetarianstypical UK recommendations include cyanocobalamin supplements providing 10-100 micrograms daily or approximately 2000 micrograms weekly
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