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Tissues were washed, 200 L of buffered saline solution was added to the apical compartment of MucilAir-HF cultures, and resistance was measured using an EVOMX volt-ohmmeter with chopstick style probes (World Precision Instruments, Stevenage, UK)

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Raw files for MS analysis of GAGs are available at GlycoPost 74 under project ID GPST000580