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2.9 Cell culture and treatment Mouse colon epithelial cells (MCECs) were cultured in high-glucose Dulbeccos Modified Eagle Medium, supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (P/S), in a humidified incubator with 5% CO 2 at 37C

10.1016/S1474-4422(20)30231-3 55 KhafagyE.-S.KameiN.NielsenE