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Transfected and parental FlpInCHO cells were maintained in DMEM supplemented with 10% heat-inactivated FBS and incubated in a humidified environment at 37 C in 5% CO 2
Hepatocyte growth factor (HGF) binds to the c-Met receptor on neurons, triggering downstream signaling cascades that promote cell survival, axon growth, and the formation of new synapses
It both directly combats the accumulation of highly-reactive oxygen species and also stimulates other defence mechanisms
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