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Tumour tissues (100500 mg) were dissected into smaller fragments to cover the filter surface of 0.22 m nylon-filtered microcentrifuge tubes (Corning, 8160), taking care to minimize mechanical cell lysis

Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae

water for injection (WFI), sterile water solution, or bacteriostatic water for injection (BWFI) may be used

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