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The other thing to keep in mind is that glutathione is acidic, so when you add it to your elution buffer it will likely make the solution more acidic

Neurology 104 (6), e213447

Dig Dis 2021;39(5):508-515

The expression of target genes was analysed via quantitative real-time PCR with a QuantaStudio 5 qPCR machine (Applied Biosystems, Thermo Fisher Scientific)

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