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Nonspecific sites on the membranes were blocked with tris-buffered saline (TBS) that contained 5% nonfat milk and 0.1% Tween 20 (TBST, Beyotime, China) for 2 h
Plasma adiponectin levels inversely correlate to clinical parameters in type 2 diabetes mellitus patients with macrovascular diseases
Importantly, mitochondrial DNA (mtDNA) mutations, which accumulate with age and HTN, perpetuate this dysfunction, rendering the vasculature more susceptible to oxidative injury
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