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But please do not let it replace the work of understanding your underlying hormonal picture, your nutrition, your sleep, and the basics of skin and scalp health
This step was followed by incubation with primary antibody overnight at 4C
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SUP-B15 cells were cultured in Iscoves modified Dulbeccos medium supplemented with 4 mM L-glutamine, 1.5 g l 1 sodium bicarbonate, 0.05 mM 2-mercaptoethanol, 80% RPMI-1640 and 20% FBS
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