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In diet-induced obesity (DIO) mouse models, 5-Amino-1MQ administered intraperitoneally produced a 7% reduction in body weight over 11 days compared to vehicle-treated controls, despite no change in food intake
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At 1 mg its occupancy sits near 1%, and liver fat still fell 51% over 48 weeks in the trial participants who started with fatty liver. Natural glucagon reaches a similar 0.5 to 5% engagement during a fast, so 1% is not an unusually small signal
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